Diagnosis by qPCR of strains (1, 2 and 4) Fusarium oxysporum f. sp. cubensis Panama disease of mosses (2015)

The research article compiles the results obtained after isolation and purification of DNA from Races 1, 2, 5 and 4 (with 4, 9, 5 and 1 isolates respectively) and 3 VCGs, amplification of the IGS region of the nuclear ribosomal operon using primers and sequencing of the amplicons obtained, and the development of qPCR primers for quantitative identification of infected isolates and tissues. High sensitivities and specificity were obtained in the diagnosis allowing detection of 1 pg of R4T and R1/R2 specific DNA, sequence similarity of the amplicons with the primers was found that could be used in early diagnosis and knowledge of the epidemiology to develop management tools.