Latin America and the Caribbean (LAC) is a megadiverse region hosting most of the world’s primary forests and significant biodiversity. However, it faces severe climate change impacts such as heat waves, droughts, hurricanes, and flooding, alongside deforestation and soil degradation. Agroforestry systems, covering between 200 and 500 million hectares in the region, offer a sustainable option to mitigate these impacts and enhance production. This special issue compiles interdisciplinary research addressing the biophysical, economic, social, and cultural aspects of agroforestry in LAC.