The complexity and interconnectedness of health challenges that threaten people, animals, plants and the environment, where they coexist, require holistic and integrated solutions with a systemic approach incorporating broader structural factors and systemic prevention measures that integrate human, animal, plant and environmental health. One Health needs continued institutionalization supported by adequate investments for increased awareness of all stakeholders, cross-sectoral competencies and capacities, joint workforce training, career pathways and opportunities, effective governance based on multi-sectoral transdisciplinary principles and appropriate legislation, stakeholder and community engagement, integration of the concept into education of related disciplines, and renewed emphasis with key interventions and collaborations at all levels to move towards a more sustainable, healthier and safer world.